[S28] Rabbit Anti-CBL (Carbamyl-lysine) AntiserumCBL30S-R1a
Specifically binds to CBL-proteins. Dilution for immunoblot and ELISA range: 1,000 to 40,000.
The antibody can be used for detection of Carbamylation in plasma, lipoproteins, and other CBL containing proteins, using for immunoassays, immunoblots, enzyme conjugation, or biotinylation.
|Storage:||Freeze-dried product should be stored refrigerated until opened. After opening, restore to suggested ml volume with distilled water. If it is not completely clear after standing for 1-2 hours at room temperature, centrifuge the product. It is stable for several weeks at 4°C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20°C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.|
*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.
Carbamylation is a spontaneous nonenzymatic modification of proteins and amino acids by urea-derived isocyanate, which is generally present in human serum and is increased in uremic patients (Kraus and Kraus, 2001).
Proteins carbamylated at lysine or the terminal protein amino acids may play an important role in Chronic renal failure subjects in the development of atherosclerosis and cardiovascular diseases (Raine et al., 1991; Culleton et al., 1999).
Culleton BF, Larson MG, Wilson PW, Evans JC, Parfrey PS, Levy D. Cardiovascular disease and mortality in a community-based cohort with mild renal insufficiency. Kidney Int 1999; 56: 2214–9.
Kraus LM, Kraus AP Jr. Carbamoylation of amino acids and proteins in uremia. Kidney Int Suppl 2001; 78: S102–7.
Raine AE, MacMahon SH, Selwood NH, Wing AJ, Brunner FP. Mortality from myocardial infarction in patients on renal replacement therapy. Nephrol Dial Transplant 1991; 6: 902.