[H01] HRP-conjugated Goat Anti-Human Albumin

[H01] HRP-conjugated Goat Anti-Human Albumin

AL10H-G1a

Academy Bio-Medical Company, Inc.

  • $252.00


Host Species: Goat
Concentration: 1 mg/ml (OD 1.35 / 280 nm)
Antigen: Human albumin
Purification: Affinity purified
Buffer: 50 mM PBS, 0.1 M NaCl, and 0.01% Thiomersal, pH 7.4.
Specificity Specifically binds to human albumin. Dilution for immunoblot & ELISA range: 1,000 to 8,000. Centrifuge before use.
Use: The antibody can be used for detection of human albumin in plasma and lipoproteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.
Storage: -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.
Form: Freeze dried powder
Stabilizer: 10 mg / ml Bovine Serum Albumin.

Reconstitution

and Storage:

Freeze-dried product should be stored refrigerated until opened. After opening, restore to suggested ml volume with distilled water. If it is not completely clear after standing for 1-2 hours at room temperature, centrifuge the product. It is stable for several weeks at 4°C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20°C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.

 

*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

 

Importance

Human albumin constitutes around 55% of the protein present in the plasma of normal healthy individuals. It is a single polypeptide chain of 585 amino acids with a molecular weight of 66 kDa.

The functions of human serum albumin include maintaining oncotic pressure; binding substances or drugs; regulating endogenous substances, metabolism; plasma buffer; antioxidant function; maintaining microvascular integrity; anticoagulant effects; inflammatory/anti-inflammatory effects; cell-signaling process. (Nicholson et al., 2000; Evans, 2002) Albumin also has functions that may play a critical role and have a great impact on different types of diseases, including hypo-albumin aemia and peripheral oedema. (Caironi et al. 2009)

Caironi, Pietro, and Luciano Gattinoni. “The clinical use of albumin: the point of view of a specialist in intensive care.” Blood Tranfusion 7.4 (2009): 259-67.

Evans, T. W. "Review Article: Albumin as a Drug-biological Effects of Albumin Unrelated to Oncotic Pressure." Alimentary Pharmacology and Therapeutics 16 (2002): 6-11.

Nicholson, J. P., M. R. Wolmarans, and G. R. Park. "The Role of Albumin in Critical Illness." British Journal of Anaesthesia 85.4 (2000): 599-610.

 

Citations

[H01] 2014 Poirier, Steve; Samami, Samaneh; Mamarbachi, Maya; Demers, Annie; Chang, Ta Yuan; Vance, Dennis E. et al. (2014): The Epigenetic Drug 5-Azacytidine Interferes with Cholesterol and Lipid Metabolism. In J. Biol. Chem. 289 (27), pp. 18736–18751. DOI: 10.1074/jbc.M114.563650.
[H01] 2008 Mezo, Adam R.; McDonnell, Kevin A.; Hehir, Cristina A. Tan; Low, Susan C.; Palombella, Vito J.; Stattel, James M. et al. (2008): Reduction of IgG in nonhuman primates by a peptide antagonist of the neonatal Fc receptor FcRn. In Proc Natl Acad Sci USA 105 (7), pp. 2337–2342. DOI: 10.1073/pnas.0708960105.

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