[G05] Goat Anti-Human Apolipoprotein E Sepharose™ 4B Gel, Academy Bio-medical Company, Inc.

[G05] Goat Anti-Human Apolipoprotein E Sepharose™ 4B Gel

50A-G1 Resin-1ml

Academy Bio-Medical Company, Inc.

  • $1,626.00


Host Species: Goat
Concentration: ≥  5 mg/ml
Source:

Polyclonal goat antibody human apo E coupled with CNBr-activated Sepharose 4B™.

Preservation:

75 mM PBS, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, 0.1mM PMSF, pH 7.3.

Specificity

Specifically binds to human apo E.

Use:

Affinity purification column.

Storage:

2-8ºC store in buffer with 0.5 mM EDTA and 0.02% NaN3, 0.1 mM PMSF. DO NOT FREEZE!

 

*Sepharose is a trademark of GE Healthcare Life Sciences, Sweden.

** These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

Importance

Apo E contains 299 amino acid residues. It is a 34-37 kDa glycosylated protein (Rall et al., 1983).

Apo E is involved with triglyceride, phospholipid, cholesteryl ester, and cholesterol transport in and out of cells and is a ligand for LDL receptors. Apo E has also been implicated in immune and nerve degeneration. It has been found to suppress lymphocyte proliferation. Late-onset familial and sporadic Alzheimer disease patients have been found to have a higher occurrence of one of the three common Apo E isoforms, Apo E4. The Apo E4 isoform has been detected in senile plaques and neurofibrillary tangles of Alzheimer disease patients. Apo E4 is associated with rapid chylomicron-remnant clearance and increased total cholesterol levels.

Rall, S C, K H Weisgraber, T L Innerarity, T P Bersot, R W Mahley, and C B Blum. "Identification of a New Structural Variant of Human Apolipoprotein E, E2(Lys146 Leads to Gln), in a Type III Hyperlipoproteinemic Subject with the E3/2 Phenotype." Journal of Clinical Investigation 72.4 (1983): 1288-297.

 

Citations

[G05] 2020

Robert, Jerome; Button, Emily B.; Martin, Emma M.; McAlary, Luke; Gidden, Zoe; Gilmour, Megan et al. (2020): Cerebrovascular amyloid Angiopathy in bioengineered vessels is reduced by high-density lipoprotein particles enriched in Apolipoprotein E. In Molecular neurodegeneration 15 (1), p. 23. DOI: 10.1186/s13024-020-00366-8.

[G05] 2018 Morton, Allyson M.; Koch, Manja; Mendivil, Carlos O.; Furtado, Jeremy D.; Tjønneland, Anne; Overvad, Kim et al. (2018): Apolipoproteins E and CIII interact to regulate HDL metabolism and coronary heart disease risk. In JCI insight 3 (4). DOI: 10.1172/jci.insight.98045.
[G05] 2010 Mendivil, Carlos O.; Zheng, Chunyu; Furtado, Jeremy; Lel, Julian; Sacks, Frank M. (2010): Metabolism of very-low-density lipoprotein and low-density lipoprotein containing apolipoprotein C-III and not other small apolipoproteins. In Arterioscler Thromb Vasc Biol. 30 (2), pp. 239–245. DOI: 10.1161/ATVBAHA.109.197830.
[G05] 2010 Zheng, Chunyu; Khoo, Christina; Furtado, Jeremy; Sacks, Frank M. (2010): Apolipoprotein C-III and the Metabolic Basis for Hypertriglyceridemia and the Dense Low-Density Lipoprotein Phenotype. In Circulation 121 (15), pp. 1722–1734. DOI: 10.1161/CIRCULATIONAHA.109.875807.
[G05] 2007 Zheng, Chunyu; Khoo, Christina; Ikewaki, Katsunori; Sacks, Frank M. (2007): Rapid turnover of apolipoprotein C-III-containing triglyceride-rich lipoproteins contributing to the formation of LDL subfractions. In J. Lipid Res. 48 (5), pp. 1190–1203. DOI: 10.1194/jlr.P600011-JLR200.
[G05] 2006 Kawakami, Akio; Aikawa, Masanori; Libby, Peter; Alcaide, Pilar; Luscinskas, Francis W.; Sacks, Frank M. (2006): Apolipoprotein CIII in apolipoprotein B lipoproteins enhances the adhesion of human monocytic cells to endothelial cells. In Circulation 113 (5), pp. 691–700. DOI: 10.1161/CIRCULATIONAHA.105.591743.
[G05] 2005 van den Elzen, Peter; Garg, Salil; León, Luis; Brigl, Manfred; Leadbetter, Elizabeth A.; Gumperz, Jenny E. et al. (2005): Apolipoprotein-mediated pathways of lipid antigen presentation. In Nature 437 (7060), pp. 906–910. DOI: 10.1038/nature04001.

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