[G02] Goat Anti-Human Apolipoprotein AII Sepharose™ 4B Gel, Academy Bio-medical Company, Inc.

[G02] Goat Anti-Human Apolipoprotein AII Sepharose™ 4B Gel

12A-G1 Resin-1ml

Academy Bio-Medical Company, Inc.

  • $1,393.00


Host Species: Goat
Concentration: ≥  5 mg/ml
Source:

Polyclonal goat antibody human apo AII coupled with CNBr-activated Sepharose 4B™.

Preservation:

75 mM PBS, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, 0.1mM PMSF, pH 7.3.

Specificity

Specifically binds to human apo AII.

Use:

Affinity purification column.

Storage:

2-8ºC store in buffer with 0.5 mM EDTA and 0.02% NaN3, 0.1 mM PMSF. DO NOT FREEZE!

 

*Sepharose is a trademark of GE Healthcare Life Sciences, Sweden.

** These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

 

Importance

Apo AII comprises 25% of HDL. It exists in human plasma as a dimer of 2 identical chains of 77 amino acid residues, joined by disulfide. The molecular weight is reported to be 8.7 kDa for a single chain (Brewer et al., 1972).

Studies on mouse reported that apo AII may be proatherogenic (Warden et al., 1993); however, case-control study in the large European Prospective Investigation demonstrated that plasma Apo AII concentrations were strongly inversely correlated with CHD events (Birjmohun et al., 2007).

Birjmohun, R. S., G. M. Dallinga-Thie, J. A. Kuivenhoven, E. S.g. Stroes, J. D. Otvos, N. J. Wareham, R. Luben, J. J.p. Kastelein, K.-T. Khaw, and S. M. Boekholdt. "Apolipoprotein A-II Is Inversely Associated With Risk of Future Coronary Artery Disease." Circulation 116 (2007): 2029-035.

Brewer, H. B., S. E. Lux, R. Ronan, and K. M. John. "Amino Acid Sequence of Human ApoLp-Gln-II (apoA-II), an Apolipoprotein Isolated from the High-Density Lipoprotein Complex." Proceedings of the National Academy of Sciences 69.5 (1972): 1304-308.

Warden, C., C. Hedrick, J. Qiao, L. Castellani, and A. Lusis. "Atherosclerosis in Transgenic Mice Overexpressing Apolipoprotein A-II." Science 261 (1993): 469-72.

 

Citations

[G02] 2017 Toth, Christopher A.; Kuklenyik, Zsuzsanna; Jones, Jeffrey I.; Parks, Bryan A.; Gardner, Michael S.; Schieltz, David M. et al. (2017): On-column trypsin digestion coupled with LC-MS/MS for quantification of apolipoproteins. In Journal of proteomics 150, pp. 258–267. DOI: 10.1016/j.jprot.2016.09.011.
[G02] 2015 Desai, Nirav K.; Ooi, Esther M.; Mitchell, Paul D.; Furtado, Jeremy; Sacks, Frank M. (2015): Metabolism of apolipoprotein A-II containing triglyceride rich ApoB lipoproteins in humans. In Atherosclerosis 241 (2), pp. 326–333. DOI: 10.1016/j.atherosclerosis.2015.05.013.
[G02] 2010 Mendivil, Carlos O.; Zheng, Chunyu; Furtado, Jeremy; Lel, Julian; Sacks, Frank M. (2010): Metabolism of very-low-density lipoprotein and low-density lipoprotein containing apolipoprotein C-III and not other small apolipoproteins. In Arterioscler Thromb Vasc Biol. 30 (2), pp. 239–245. DOI: 10.1161/ATVBAHA.109.197830.

 


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