[A23] Rabbit Anti-4 HNE (4 Hydroxynonenal) Polyclonal Antibody, 30% glycerolHNE40A-R1a
|Concentration:||1 mg/ml (OD 1.35 / 280 nm)|
|Buffer:||75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2, 30% glycerol|
Specifically binds to 4-HNE modified proteins. Dilution for immunoblot and ELISA range: 1,000 to 8,000. (A slight amount of precipitation may have occurred during storage due to the natural properties of these antibodies; please centrifuge before use.).
The antibody can be used for detection of of 4-HNE modification in plasma, lipoproteins, and other proteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.
|Storage:||-20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.|
Modifications on lysine residues, with formation of carboxylmethyl-lysine (CML), malondialdehyde (MDA) and hexitol-lysine are advanced glycation end-products (AGE), and the coupling with reactive aldehyde compounds, such as 4-hydroxynonenal (4-HNE) may appear from lipid oxidation (Guichardant et al., 1998). These modifications feature the oxidative byproducts which react with NH2 groups and form Schiff-base adducts (Mark et al., 1996). LDL treated with HNE or oxidatively modified by Cu++ or by cultured endothelial cells give rise to Michael addition-type HNE adducts that are recognized by HNE-specific antibodies.
Guichardant M, Taibi-Tronche P, Fay LB, Lagarde M. Covalent modifications of aminophospholipids by 4-hydroxynonenal. Free Radic Biol Med. 1998 Dec;25(9):1049-56.
Mark S. Bolgar, Chao-Yuh Yang, and Simon J. Gaskell, First Direct Evidence for Lipid/Protein Conjugation in Oxidized Human Low Density Lipoprotein (LDL), JBC, 271: 27999-28001 (1996).
|[A22][A23]||2013||Arashiki, Nobuto; Kimata, Naoki; Manno, Sumie; Mohandas, Narla; Takakuwa, Yuichi (2013): Membrane peroxidation and methemoglobin formation are both necessary for band 3 clustering: mechanistic insights into human erythrocyte senescence. In Biochemistry 52 (34), pp. 5760–5769. DOI: 10.1021/bi400405p.|
|[A22][A23]||2010||Yang, Xiaoling; Bosoi, Cristina R.; Jiang, Wenlei; Tremblay, Mélanie; Rose, Christopher F. (2010): Portacaval anastomosis-induced hyperammonemia does not lead to oxidative stress. In Metab Brain Dis 25 (1), pp. 11–15. DOI: 10.1007/s11011-010-9174-1.|