[A16] Goat Anti-MDA (Malondialdehyde) Polyclonal Antibody - AcademyBiomed

[A16] Goat Anti-MDA (Malondialdehyde) Polyclonal Antibody


Academy Bio-Medical Company, Inc.

  • $295.00

Host Species: Goat
Concentration: 1 mg/ml (OD 1.35 / 280 nm)
Antigen: MDA-KLH
Purification: Affinity purified
Buffer: 75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2
Specificity Specifically binds to MDA-LDL and other MDA modified proteins. Dilution for Immunoblot & ELISA range: 1,000 to 20,000. (A slight amount of precipitation may have occurred during storage due to the natural properties of these antibodies; please centrifuge before use.)
Use: The antibody can be used for detection of MDA in plasma, lipoproteins and other MDA modified proteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.
Storage: -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.


*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.


Oxidative damage includes oxidative modification of cellular macromolecules, induction of cell death by apoptosis or necrosis, as well as structural tissue damage. Of the many biological targets of oxidative stress, lipids are the most involved class of biomolecules. Lipid oxidation gives rise to a number of secondary products of polyunsaturated fatty acid peroxidation. Malondialdehyde (MDA) is the principal and most studied product. Consistent evidence reveals the reaction between MDA and cellular macromolecules such as proteins, RNA and DNA (Valenzuela, 1991).

Numerous experiments have shown that MDA readily modifies proteins (Nair, 1986). MDA reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine which may be mutagenic and these can be quantified in several human tissues (Marnett, 1999).This aldehyde is a highly toxic molecule and should be considered as a marker of lipid peroxidation. The interaction with DNA and proteins has often been referred to as potentially mutagenic and atherogenic (Rio et al., 2005).

L.J. Marnett, Lipid peroxidation‐DNA damage by malondialdehyde, Mutat Res, 424 (1999), pp. 83–95

V. Nair, C.S. Cooper, D.E. Vietti, G.A. Turner, The chemistry of lipid peroxidation metabolites: crosslinking reactions of malondialdehyde, Lipids, 21 (1986), pp. 6–10

Rio, Daniele Del, Amanda J. Stewart, and Nicoletta Pellegrini. "A Review of Recent Studies on Malondialdehyde as Toxic Molecule and Biological Marker of Oxidative Stress." Nutrition, Metabolism and Cardiovascular Diseases 15.4 (2005): 316-28. 

A. Valenzuela, The biological significance of malondialdehyde determination in the assessment of tissue oxidative stress, Life Sci, 48 (1991), pp. 301–309



[A16][A17] 2020 Busquets, Sílvia; Pérez-Peiró, Maria; Salazar-Degracia, Anna; Argilés, Josep M.; Serpe, Roberto; Rojano-Toimil, Alba et al. (2020): Differential structural features in soleus and gastrocnemius of carnitine-treated cancer cachectic rats. Journal of Cellular Physiology 235 (1), pp. 526–537. DOI: 10.1002/jcp.28992.
[A16][A17] 2019 Himori, Koichi; Tatebayashi, Daisuke; Ashida, Yuki; Yamada, Takashi (2019): Eccentric training enhances the αB-crystallin binding to the myofibrils and prevents skeletal muscle weakness in adjuvant-induced arthritis rat. Journal of Applied Physiology 127 (1), pp. 71–80. DOI: 10.1152/japplphysiol.00102.2019.
[A16][A17] 2019 Oniki, Takahiro; Teshima, Yasushi; Nishio, Satoru; Ishii, Yumi; Kira, Shintaro; Abe, Ichitaro et al. (2019): Hyponatraemia aggravates cardiac susceptibility to ischaemia/reperfusion injury. International Journal of Experimental Pathology 100 (5-6), pp. 350–358. DOI: 10.1111/iep.12338.
[A16][A17] 2019 Rahman, Mizanur; Steuer, Johnny; Gillgren, Peter; Végvári, Ákos; Liu, Anquan; Frostegård, Johan (2019): Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell-Mediated Mechanism. JACC. Basic to Translational Science 4 (4), pp. 480–494. DOI: 10.1016/j.jacbts.2019.03.009.
[A16][A17] 2019 Barreiro, Esther; Salazar‐Degracia, Anna; Sancho‐Muñoz, Antonio; Gea, Joaquim (2019): Endoplasmic reticulum stress and unfolded protein response profile in quadriceps of sarcopenic patients with respiratory diseases. In Journal of cellular physiology 234 (7), pp. 11315–11329. DOI: 10.1002/jcp.27789.
[A16][A17] 2018 Barreiro, Esther; Puig-Vilanova, Ester; Salazar-Degracia, Anna; Pascual-Guardia, Sergi; Casadevall, Carme; Gea, Joaquim (2018): The phosphodiesterase-4 inhibitor roflumilast reverts proteolysis in skeletal muscle cells of patients with COPD cachexia. In Journal of Applied Physiology 125 (2), pp. 287–303. DOI: 10.1152/japplphysiol.00798.2017.
[A16][A17] 2018 Yamada, Ryotaro; Himori, Koichi; Tatebayashi, Daisuke; Ashida, Yuki; Ikezaki, Kazumi; Miyata, Hirohumi et al. (2018): Preconditioning contractions prevent the delayed onset of myofibrillar dysfunction after damaging eccentric contractions. In J Physiol 596 (18), pp. 4427–4442. DOI: 10.1113/JP276026.
[A16][A17] 2017 Salazar-Degracia, Anna; Busquets, Sílvia; Argilés, Josep M.; López-Soriano, Francisco J.; Barreiro, Esther (2017): Formoterol attenuates increased oxidative stress and myosin protein loss in respiratory and limb muscles of cancer cachectic rats. In PeerJ 5 (Suppl 1), e4109. DOI: 10.7717/peerj.4109.
[A16][A17] 2016 Salazar-Degracia, Anna; Blanco, David; Vilà-Ubach, Mònica; Biurrun, Gabriel de; Solórzano, Carlos Ortiz de; Montuenga, Luis M.; Barreiro, Esther (2016): Phenotypic and metabolic features of mouse diaphragm and gastrocnemius muscles in chronic lung carcinogenesis: influence of underlying emphysema. In Journal of Translational Medicine 14 (1), p. 244. DOI: 10.1186/s12967-016-1003-9.
[A16][A17] 2015 Aoki, Kohei; Teshima, Yasushi; Kondo, Hidekazu; Saito, Shotaro; Fukui, Akira; Fukunaga, Naoya et al. (2015): Role of Indoxyl Sulfate as a Predisposing Factor for Atrial Fibrillation in Renal Dysfunction. In Journal of the American Heart Association 4 (10), e002023. DOI: 10.1161/JAHA.115.002023.
[A16][A17] 2015 El-Mas, Mahmoud M.; Abdel-Rahman, Abdel A. (2015): Estrogen modulation of the ethanol-evoked myocardial oxidative stress and dysfunction via DAPK3/Akt/ERK activation in male rats. In Toxicology and Applied Pharmacology 287 (3), pp. 284–292. DOI: 10.1016/j.taap.2015.06.015.
[A16][A17] 2015 Yamada, Takashi; Abe, Masami; Lee, Jaesik; Tatebayashi, Daisuke; Himori, Koichi; Kanzaki, Keita et al. (2015): Muscle dysfunction associated with adjuvant-induced arthritis is prevented by antioxidant treatment. In Skeletal Muscle 5, p. 20. DOI: 10.1186/s13395-015-0045-7.
[A16][A17] 2014 El-Mas, Mahmoud M.; Abdel-Rahman, Abdel A. (2014): Nongenomic effects of estrogen mediate the dose-related myocardial oxidative stress and dysfunction caused by acute ethanol in female rats. In American journal of physiology. Endocrinology and Metabolism 306 (7), E740-7. DOI: 10.1152/ajpendo.00465.2013.
[A16][A17] 2014 Ibrahim, Badr M.; Fan, Ming; Abdel-Rahman, Abdel A. (2014): Oxidative Stress and Autonomic Dysregulation Contribute to the Acute Time-Dependent Myocardial Depressant Effect of Ethanol in Conscious Female Rats. In Alcoholism, Clinical and Experimental Research 38 (5), pp. 1205–1215. DOI: 10.1111/acer.12363.
[A16][A17] 2014 Okutsu, Mitsuharu; Call, Jarrod A.; Lira, Vitor A.; Zhang, Mei; Donet, Jean A.; French, Brent A. et al. (2014): Extracellular superoxide dismutase ameliorates skeletal muscle abnormalities, cachexia, and exercise intolerance in mice with congestive heart failure. In Circulation. Heart failure 7 (3), pp. 519–530. DOI: 10.1161/CIRCHEARTFAILURE.113.000841.
[A16][A17] 2014 Saito, Shotaro; Teshima, Yasushi; Fukui, Akira; Kondo, Hidekazu; Nishio, Satoru; Nakagawa, Mikiko et al. (2014): Glucose fluctuations increase the incidence of atrial fibrillation in diabetic rats. In Cardiovascular Research 104 (1), pp. 5–14. DOI: 10.1093/cvr/cvu176.
[A16][A17] 2014 Sáyago-Ayerdi, Sonia G.; Mateos, Raquel; Ortiz-Basurto, Rosa I.; Largo, Carlota; Serrano, José; Granado-Serrano, Ana Belén et al. (2014): Effects of consuming diets containing Agave tequilana dietary fibre and jamaica calyces on body weight gain and redox status in hypercholesterolemic rats. In Food Chemistry 148, pp. 54–59. DOI: 10.1016/j.foodchem.2013.10.004.
[A16][A17] 2014 Slot, Ilse G. M.; van den Borst, Bram; Hellwig, Valéry A. C. V.; Barreiro, Esther; Schols, Annemie M. W. J.; Gosker, Harry R. (2014): The muscle oxidative regulatory response to acute exercise is not impaired in less advanced COPD despite a decreased oxidative phenotype. In PLoS ONE 9 (2), e90150. DOI: 10.1371/journal.pone.0090150.
[A16][A17] 2014 Yang, Tzu-Ching; Chen, Yi-Jie; Chang, Shwu-Fen; Chen, Chu-Huang; Chang, Po-Yuan; Lu, Shao-Chun (2014): Malondialdehyde mediates oxidized LDL-induced coronary toxicity through the Akt-FGF2 pathway via DNA methylation. In Journal of Biomedical Science 21, p. 11. DOI: 10.1186/1423-0127-21-11.
[A16][A17] 2014 Zhu, Xinmei; Ng, Hang Pong; Lai, Yen-Chun; Craigo, Jodi K.; Nagilla, Pruthvi S.; Raghani, Pooja; Nagarajan, Shanmugam (2014): Scavenger Receptor Function of Mouse Fcγ Receptor III Contributes to Progression of Atherosclerosis in Apolipoprotein E Hyperlipidemic Mice. In J.I. 193 (5), pp. 2483–2495. DOI: 10.4049/jimmunol.1303075.
[A16][A17] 2013 Arashiki, Nobuto; Kimata, Naoki; Manno, Sumie; Mohandas, Narla; Takakuwa, Yuichi (2013): Membrane peroxidation and methemoglobin formation are both necessary for band 3 clustering: mechanistic insights into human erythrocyte senescence. In Biochemistry 52 (34), pp. 5760–5769. DOI: 10.1021/bi400405p.
[A16][A17] 2013 Serrano, José C. E.; Gonzalo-Benito, Hugo; Jové, Mariona; Fourcade, Stéphane; Cassanyé, Anna; Boada, Jordi et al. (2013): Dietary intake of green tea polyphenols regulates insulin sensitivity with an increase in AMP-activated protein kinase α content and changes in mitochondrial respiratory complexes. In Molecular Nutrition & Food Research 57 (3), pp. 459–470. DOI: 10.1002/mnfr.201200513.
[A16][A17] 2013 van den Borst, Bram; Slot, Ilse G. M.; Hellwig, Valéry A. C. V.; Vosse, Bettine A. H.; Kelders, Marco C. J. M.; Barreiro, Esther et al. (2013): Loss of quadriceps muscle oxidative phenotype and decreased endurance in patients with mild-to-moderate COPD. In Journal of Applied Physiology 114 (9), pp. 1319–1328. DOI: 10.1152/japplphysiol.00508.2012.
[A16][A17] 2012 Barreiro, Esther; del Puerto-Nevado, Laura; Puig-Vilanova, Ester; Pérez-Rial, Sandra; Sánchez, Francisco; Martínez-Galán, Lourdes et al. (2012): Cigarette smoke-induced oxidative stress in skeletal muscles of mice. In Respiratory Physiology & Neurobiology 182 (1), pp. 9–17. DOI: 10.1016/j.resp.2012.02.001.
[A16][A17] 2012 Fukunaga, Naoya; Takahashi, Naohiko; Hagiwara, Satoshi; Kume, Osamu; Fukui, Akira; Teshima, Yasushi et al. (2012): Establishment of a model of atrial fibrillation associated with chronic kidney disease in rats and the role of oxidative stress. In Heart Rhythm 9 (12), pp. 2023–2031. DOI: 10.1016/j.hrthm.2012.08.019.
[A16][A17] 2011 Andersson, Daniel C.; Fauconnier, Jérémy; Yamada, Takashi; Lacampagne, Alain; Zhang, Shi-Jin; Katz, Abram; Westerblad, Håkan (2011): Mitochondrial production of reactive oxygen species contributes to the β-adrenergic stimulation of mouse cardiomycytes. In J Physiol 589 (7), pp. 1791–1801. DOI: 10.1113/jphysiol.2010.202838.
[A16][A17] 2011 André, Lucas; Gouzi, Fares; Thireau, Jérôme; Meyer, Gregory; Boissiere, Julien; Delage, Martine et al. (2011): Carbon monoxide exposure enhances arrhythmia after cardiac stress: involvement of oxidative stress. In Basic Res Cardiol 106 (6), pp. 1235–1246. DOI: 10.1007/s00395-011-0211-y.
[A16][A17] 2011 Ferrer, Isidro; Carmona, Margarita; Blanco, Rosa; Recio, Maria; San Segundo, R. (2011): Gerstmann-Straüssler-Scheinker PRNP P102L-129V mutation. In Translational Neuroscience 2 (1), p. 1197. DOI: 10.2478/s13380-011-0001-x.
[A16][A17] 2011 Tolfsen, C. C.; Baker, N.; Kreibich, C.; Amdam, G. V. (2011): Flight restriction prevents associative learning deficits but not changes in brain protein-adduct formation during honeybee ageing. In Journal of Experimental Biology 214 (8), pp. 1322–1332. DOI: 10.1242/jeb.049155.
[A16][A17] 2010 Casós, Kelly; Zaragozá, María C.; Zarkovic, Neven; Zarkovic, Kamelija; Andrisic, Luka; Portero-Otín, Manuel et al. (2010): A fish-oil-rich diet reduces vascular oxidative stress in apoE(-/-) mice. In Free Radical Research 44 (7), pp. 821–829. DOI: 10.3109/10715762.2010.485992.
[A16][A17] 2010 Cholewa, Jill; Nikolic, Dejan; Post, Steven R. (2010): Regulation of class A scavenger receptor-mediated cell adhesion and surface localization by PI3K: identification of a regulatory cytoplasmic motif. In Journal of Leukocyte Biology 87 (3), pp. 443–449. DOI: 10.1189/jlb.0509318.
[A16][A17] 2010 Yamada, Takashi; Zhang, Shi-Jin; Westerblad, Hakan; Katz, Abram (2010): {beta}-Hydroxybutyrate inhibits insulin-mediated glucose transport in mouse oxidative muscle. In American Journal of Physiology-Endocrinology and Metabolism 299 (3), E364-73. DOI: 10.1152/ajpendo.00142.2010.
[A16][A17] 2009 Aydin, Jan; Andersson, Daniel C.; Hänninen, Sandra L.; Wredenberg, Anna; Tavi, Pasi; Park, Chan Bae et al. (2009): Increased mitochondrial Ca2+ and decreased sarcoplasmic reticulum Ca2+ in mitochondrial myopathy. In Human Molecular Genetics 18 (2), pp. 278–288. DOI: 10.1093/hmg/ddn355.
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[A16][A17] 2008 Bustamante, Víctor; Casanova, Juan; López de Santamaría, Elena; Mas, Sandra; Sellarés, Jacobo; Gea, Joaquim et al. (2008): Redox balance following magnetic stimulation training in the quadriceps of patients with severe COPD. In Free Radical Research 42 (11-12), pp. 939–948. DOI: 10.1080/10715760802555569.
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[A16][A17] 2008 Wallace, Sunny; Vaughn, Katherine; Stewart, Bradford W.; Viswanathan, Tito; Clausen, Edgar; Nagarajan, Shanmugam; Carrier, Danielle Julie (2008): Milk Thistle Extracts Inhibit the Oxidation of Low-Density Lipoprotein (LDL) and Subsequent Scavenger Receptor-Dependent Monocyte Adhesion. In J. Agric. Food Chem. 56 (11), pp. 3966–3972. DOI: 10.1021/jf703694u.
[A16][A17] 2007 Barreiro, Esther; Nowinski, Adam; Gea, Joaquim; Sliwinski, Pawel (2007): Oxidative stress in the external intercostal muscles of patients with obstructive sleep apnoea. In Thorax 62 (12), pp. 1095–1101. DOI: 10.1136/thx.2006.069963.
[A16][A17] 2007 Choksi, Kashyap B.; Nuss, Jonathan E.; Boylston, William H.; Rabek, Jeffrey P.; Papaconstantinou, John (2007): Age-related increases in oxidatively damaged proteins of mouse kidney mitochondrial electron transport chain complexes. In Free Radical Biology and Medicine 43 (10), pp. 1423–1438. DOI: 10.1016/j.freeradbiomed.2007.07.027.
[A16][A17] 2007 Janué, Anna; Olivé, Montse; Ferrer, Isidre (2007): Oxidative stress in desminopathies and myotilinopathies: a link between oxidative damage and abnormal protein aggregation. In Brain Pathology 17 (4), pp. 377–388. DOI: 10.1111/j.1750-3639.2007.00087.x.
[A16][A17] 2007 Nagarajan, Shanmugam (2007): Anti-OxLDL IgG blocks OxLDL interaction with CD36, but promotes FcgammaR, CD32A-dependent inflammatory cell adhesion. In Immunology Letters 108 (1), pp. 52–61. DOI: 10.1016/j.imlet.2006.09.008.
[A16][A17] 2007 Nikolic, Dejan M.; Cholewa, Jill; Gass, Cecelia; Gong, Ming C.; Post, Steven R. (2007): Class A scavenger receptor-mediated cell adhesion requires the sequential activation of Lyn and PI3-kinase. In American journal of physiology. Cell Physiology 292 (4), C1450-8. DOI: 10.1152/ajpcell.00401.2006.
[A16][A17] 2007 Nikolic, Dejan M.; Gong, Ming C.; Turk, John; Post, Steven R. (2007): Class A scavenger receptor-mediated macrophage adhesion requires coupling of calcium-independent phospholipase A(2) and 12/15-lipoxygenase to Rac and Cdc42 activation. In J. Biol. Chem. 282 (46), pp. 33405–33411. DOI: 10.1074/jbc.M704133200.
[A16][A17] 2007 Ping, Xiao-Du; Harris, Frank L.; Brown, Lou Ann S.; Gauthier, Theresa W. (2007): In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure. In Alcoholism, Clinical and Experimental Research 31 (2), pp. 308–316. DOI: 10.1111/j.1530-0277.2006.00306.x.
[A16][A17] 2007 Sellares, Jacobo; Mas, Sandra; Melo, Esther; Sánchez, Francesc; Marin, Judith; Gea, Joaquim; Barreiro, Esther (2007): Oxidative stress time course in the rat diaphragm after freezing-thawing cycles. In Respiratory Physiology & Neurobiology 155 (2), pp. 156–166. DOI: 10.1016/j.resp.2006.05.008.
[A16][A17] 2007 Toroser, Dikran; Orr, William C.; Sohal, Rajindar S. (2007): Carbonylation of mitochondrial proteins in Drosophila melanogaster during aging. In Biochemical and Biophysical Research Communications 363 (2), pp. 418–424. DOI: 10.1016/j.bbrc.2007.08.193.
[A16][A17] 2006 Barreiro, Esther; Coronell, Carlos; Laviña, Barbara; Ramírez-Sarmiento, Alba; Orozco-Levi, Mauricio; Gea, Joaquim (2006): Aging, sex differences, and oxidative stress in human respiratory and limb muscles. In Free Radical Biology and Medicine 41 (5), pp. 797–809. DOI: 10.1016/j.freeradbiomed.2006.05.027.
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[A16][A17] 2005 Barreiro, Esther; La Puente, Beatriz de; Busquets, Sílvia; López-Soriano, Francisco J.; Gea, Joaquim; Argilés, Josep M. (2005): Both oxidative and nitrosative stress are associated with muscle wasting in tumour-bearing rats. In FEBS Letters 579 (7), pp. 1646–1652. DOI: 10.1016/j.febslet.2005.02.017.
[A16][A17] 2005 Dalfó, Esther; Portero-Otín, Manuel; Ayala, Victoria; Martínez, Anna; Pamplona, Reinald; Ferrer, Isidre (2005): Evidence of oxidative stress in the neocortex in incidental Lewy body disease. In Journal of Neuropathology and Experimental Neurology 64 (9), pp. 816–830. DOI: 10.1097/01.jnen.0000179050.54522.5a.
[A16][A17] 2005 Gauthier, Theresa W.; Ping, Xiao-Du; Harris, Frank L.; Wong, Michael; Elbahesh, Husni; Brown, Lou Ann S. (2005): Fetal alcohol exposure impairs alveolar macrophage function via decreased glutathione availability. In Pediatric Research 57 (1), pp. 76–81. DOI: 10.1203/01.PDR.0000149108.44152.D3.
[A16][A17] 2005 Pamplona, Reinald; Dalfó, Esther; Ayala, Victòria; Bellmunt, Maria Josep; Prat, Joan; Ferrer, Isidre; Portero-Otín, Manuel (2005): Proteins in human brain cortex are modified by oxidation, glycoxidation, and lipoxidation. Effects of Alzheimer disease and identification of lipoxidation targets. In J. Biol. Chem. 280 (22), pp. 21522–21530. DOI: 10.1074/jbc.M502255200.
[A16][A17] 2005 Pamplona, Reinald; Portero-Otín, Manuel; Sanz, Alberto; Ayala, Victoria; Vasileva, Ekaterina; Barja, Gustavo (2005): Protein and lipid oxidative damage and complex I content are lower in the brain of budgerigar and canaries than in mice. Relation to aging rate. In Age (Dordrecht, Netherlands) 27 (4), pp. 267–280. DOI: 10.1007/s11357-005-4562-x.
[A16][A17] 2005 Yarian, Connie S.; Rebrin, Igor; Sohal, Rajindar S. (2005): Aconitase and ATP synthase are targets of malondialdehyde modification and undergo an age-related decrease in activity in mouse heart mitochondria. In Biochemical and Biophysical Research Communications 330 (1), pp. 151–156. DOI: 10.1016/j.bbrc.2005.02.135.
[A16][A17] 2004 Wen, Jian-Jun; Vyatkina, Galina; Garg, Nisha (2004): Oxidative damage during chagasic cardiomyopathy development: role of mitochondrial oxidant release and inefficient antioxidant defense. In Free Radical Biology and Medicine 37 (11), pp. 1821–1833. DOI: 10.1016/j.freeradbiomed.2004.08.018.
[A16][A17] 2004 Ye, Gang; Metreveli, Naira S.; Donthi, Rajakumar V.; Xia, Shen; Xu, Ming; Carlson, Edward C.; Epstein, Paul N. (2004): Catalase protects cardiomyocyte function in models of type 1 and type 2 diabetes. In Diabetes 53 (5), pp. 1336–1343. DOI: 10.2337/diabetes.53.5.1336.

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