[A09] Rabbit Anti-Human Apolipoprotein CI Polyclonal Antibody

[A09] Rabbit Anti-Human Apolipoprotein CI Polyclonal Antibody

31A-R1a

Academy Bio-Medical Company, Inc.

  • $295.00


Host Species: Rabbit
Concentration: 1 mg/ml (OD 1.35 / 280 nm)
Antigen: Human Apolipoprotein CI
Purification: Affinity purified
Buffer: 75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2
Specificity Specifically binds to human apo CI. Dilution for immunoblot and ELISA range: 1,000 to 8,000.
Use: The antibody can be used for detection of apo CI in plasma and lipoproteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.
Storage: -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.

 

*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

 

Importance

ApoCI contains 57 amino acid residues and the m.w. is 6.6 kDa (Jackson et al., 1974).

ApoCI has been found to activate LCAT (Liu and Subbaiah 1993). Apo CI is an inhibitor of lipoprotein binding to the LDL receptor, LDL receptor-related protein, and VLDL receptor. Apo CI is also an inhibitor of the plasma cholesteryl ester transfer protein and of fatty acid uptake into tissues (Shachter, 2001). This inhibition role can potentially regulate several lipase enzymes (Poensgen, 1990, Conde-Knape et al., 2002; Berbee et al., 2005.)

Berbee J.F., C.C. van der Hoogt, D. Sundararaman, L.M. Havekes, and P.C. Rensen. “Severe hypertriglyceridemia in human APOC1 transgenic mice is caused by apoC-I-induced inhibition of LPL.” J. Lipid. Res. 46 (2005) 297-306.

Conde-Knape K., A. Bensadoun, J.H. Sobel. J.S. Cohn, and N.S. Shachter. “Overexpression of apoC-I in apoE-null mice: severe hypertriglyceridemia due to inhibition of hepatic lipase” J. Lipid Res. 43 (2002): 2136-2145.

Jackson R.L.,  J. T.  Sparrow, H. N. Baker, J.D. Morrisett, O. D. Taunton, and A. M. Jr. Gotto. “The primary structure of apolipoprotein-serine.” J. Biol. Chem. 249.16 (1974): 5308-13.

Liu, M. and P.V. Subbaiah. “Activation of plasma lysolecithin acyltransferase reaction by apolipoproteins A-I, C-I and E.” Biochim. Biophys. Acta. 1168 (1993):  144-152.

Poensgen, J., “Apolipoprotein C-1 inhibits the hydrolysis by phospholipase A2 of phospholipids in liposomes and cell membranes” Biochim. Biophys. Acta. 1042 (1990): 188-192.

Shachter, Neil S., “Apolipoproteins C-I and C-III as important modulators of lipoprotein metabolism” Current Opinion in Lipidology 12(3): 297-304.

 

Citations

[A08][A09] 2017 Bus, Pascal; Pierneef, Louise; Bor, Rosalie; Wolterbeek, Ron; van Es, Leendert A.; Rensen, Patrick Cn et al. (2017): Apolipoprotein C-I plays a role in the pathogenesis of glomerulosclerosis. In J. Pathol 241 (5), pp. 589–599. DOI: 10.1002/path.4859.
[A08][A09] 2017 Wassef, Hanny; Bissonnette, Simon; Dufour, Robert; Davignon, Jean; Faraj, May (2017): Enrichment of Triglyceride-Rich Lipoproteins with Apolipoprotein C-I Is Positively Associated with Their Delayed Plasma Clearance Independently of Other Transferable Apolipoproteins in Postmenopausal Overweight and Obese Women. In J. Nutr. 147 (5), pp. 754–762. DOI: 10.3945/jn.116.242750.
[A08][A09] 2015 Trenchevska, Olgica; Schaab, Matthew R.; Nelson, Randall W.; Nedelkov, Dobrin (2015): Development of multiplex mass spectrometric immunoassay for detection and quantification of apolipoproteins C-I, C-II, C-III and their proteoforms. In Methods (San Diego, Calif.) 81, pp. 86–92. DOI: 10.1016/j.ymeth.2015.02.020.
[A08][A09] 2015 Yassine, Hussein N.; Trenchevska, Olgica; Ramrakhiani, Ambika; Parekh, Aarushi; Koska, Juraj; Walker, Ryan W. et al. (2015): The Association of Human Apolipoprotein C-III Sialylation Proteoforms with Plasma Triglycerides. In PLoS ONE 10 (12), e0144138. DOI: 10.1371/journal.pone.0144138.
[A08][A09] 2014 Zvintzou, Evangelia; Skroubis, George; Chroni, Angelika; Petropoulou, Peristera-Ioanna; Gkolfinopoulou, Christina; Sakellaropoulos, George et al. (2014): Effects of bariatric surgery on HDL structure and functionality: results from a prospective trial. In Journal of Clinical Lipidology 8 (4), pp. 408–417. DOI: 10.1016/j.jacl.2014.05.001.
[A08][A09] 2013 Takinami, Yoshihiko; Yoshimatsu, Shinya; Uchiumi, Takaoki; Toyosaki-Maeda, Tomoko; Morita, Atsushi; Ishihara, Takeshi et al. (2013): Identification of Potential Prognostic Markers for Knee Osteoarthritis by Serum Proteomic Analysis. In BiomarkInsights 8, BMI.S11966. DOI: 10.4137/BMI.S11966.
[A08][A09] 2012 Cudaback, Eiron; Li, Xianwu; Yang, Yue; Yoo, Thomas; Montine, Kathleen S.; Craft, Suzanne et al. (2012): Apolipoprotein C-I is an APOE genotype-dependent suppressor of glial activation. In Journal of neuroinflammation 9, p. 192. DOI: 10.1186/1742-2094-9-192.
[A08][A09] 2012 Wassef, Hanny; Salem, Huda; Bissonnette, Simon; Baass, Alexis; Dufour, Robert; Davignon, Jean; Faraj, May (2012): White Adipose Tissue Apolipoprotein C-I Secretion in Relation to Delayed Plasma Clearance of Dietary Fat in Humans. In Arterioscler Thromb Vasc Biol. 32 (11), pp. 2785–2793. DOI: 10.1161/ATVBAHA.112.300306.
[A08][A09] 2010 Lahiry, Piya; Cao, Henian; Ban, Matthew R.; Pollex, Rebecca L.; Mamakeesick, Mary; Zinman, Bernard et al. (2010): APOC1 T45S polymorphism is associated with reduced obesity indices and lower plasma concentrations of leptin and apolipoprotein C-I in aboriginal Canadians. In J. Lipid Res. 51 (4), pp. 843–848. DOI: 10.1194/jlr.P002014.
[A08][A09] 2009 Barlage, Stefan; Gnewuch, Carsten; Liebisch, Gerhard; Wolf, Zsuzsanna; Audebert, Franz-Xaver; Glück, Thomas et al. (2009): Changes in HDL-associated apolipoproteins relate to mortality in human sepsis and correlate to monocyte and platelet activation. In Intensive care medicine 35 (11), pp. 1877–1885. DOI: 10.1007/s00134-009-1609-y.
[A08][A09] 2008 Abildayeva, Karlygash; Berbée, Jimmy F. P.; Blokland, Arjan; Jansen, Paula J.; Hoek, Frans J.; Meijer, Onno et al. (2008): Human apolipoprotein C-I expression in mice impairs learning and memory functions. In J. Lipid Res. 49 (4), pp. 856–869. DOI: 10.1194/jlr.M700518-JLR200.
[A08][A09] 2008 Berbée, Jimmy F. P.; Mooijaart, Simon P.; Craen, Anton J. M. de; Havekes, Louis M.; van Heemst, Diana; Rensen, Patrick C. N.; Westendorp, Rudi G. J. (2008): Plasma apolipoprotein CI protects against mortality from infection in old age. In The journals of gerontology. Series A, Biological sciences and medical sciences 63 (2), pp. 122–126. DOI: 10.1093/gerona/63.2.122.
[A08][A09] 2008 Christoffersen, Christina; Jauhiainen, Matti; Moser, Markus; Porse, Bo; Ehnholm, Christian; Boesl, Michael et al. (2008): Effect of Apolipoprotein M on High Density Lipoprotein Metabolism and Atherosclerosis in Low Density Lipoprotein Receptor Knock-out Mice. In J. Biol. Chem. 283 (4), pp. 1839–1847. DOI: 10.1074/jbc.M704576200.
[A08][A09] 2008 He, Yubin; Greene, Diane J.; Kinter, Michael; Morton, Richard E. (2008): Control of cholesteryl ester transfer protein activity by sequestration of lipid transfer inhibitor protein in an inactive complex. In J. Lipid Res. 49 (7), pp. 1529–1537. DOI: 10.1194/jlr.M800087-JLR200.
[A08][A09] 2008 Li, Li; Thompson, Patricia A.; Kitchens, Richard L. (2008): Infection induces a positive acute phase apolipoprotein E response from a negative acute phase gene: role of hepatic LDL receptors. In J. Lipid Res. 49 (8), pp. 1782–1793. DOI: 10.1194/jlr.M800172-JLR200.
[A08][A09] 2007 Steen, Henning; Kolmakova, Antonina; Stuber, Matthias; Rodriguez, E. Rene; Gao, Fabao; Chatterjee, Subroto; Lima, Joao A. (2007): MRI visualized neo-intimal dissection and co-localization of novel apoptotic markers apolipoprotein C-1, ceramide and caspase-3 in a Watanabe hyperlipidemic rabbit model. In Atherosclerosis 191 (1), pp. 82–89. DOI: 10.1016/j.atherosclerosis.2006.05.022.
[A08][A09] 2007 Westerterp, Marit; Berbée, Jimmy F. P.; Delsing, Dianne J. M.; Jong, Miek C.; Gijbels, Marion J. J.; Dahlmans, Vivian E. H. et al. (2007): Apolipoprotein C-I binds free fatty acids and reduces their intracellular esterification. In J. Lipid Res. 48 (6), pp. 1353–1361. DOI: 10.1194/jlr.M700024-JLR200.
[A08][A09] 2006 Göbel, Thomas; Vorderwülbecke, Sonja; Hauck, Katarzyna; Fey, Holger; Häussinger, Dieter; Erhardt, Andreas (2006): New multi protein patterns differentiate liver fibrosis stages and hepatocellular carcinoma in chronic hepatitis C serum samples. In World journal of gastroenterology 12 (47), pp. 7604–7612. DOI: 10.3748/wjg.v12.i47.7604.
[A08][A09] 2006 Gonçalves, A.; Esterni, B.; Bertucci, F.; Sauvan, R.; Chabannon, C.; Cubizolles, M. et al. (2006): Postoperative serum proteomic profiles may predict metastatic relapse in high-risk primary breast cancer patients receiving adjuvant chemotherapy. In Oncogene 25 (7), pp. 981–989. DOI: 10.1038/sj.onc.1209131;
[A08][A09] 2006 Kawakami, Akio; Aikawa, Masanori; Libby, Peter; Alcaide, Pilar; Luscinskas, Francis W.; Sacks, Frank M. (2006): Apolipoprotein CIII in apolipoprotein B lipoproteins enhances the adhesion of human monocytic cells to endothelial cells. In Circulation 113 (5), pp. 691–700. DOI: 10.1161/CIRCULATIONAHA.105.591743.
[A08][A09] 2006 Thompson, Patricia A.; Kitchens, Richard L. (2006): Native high-density lipoprotein augments monocyte responses to lipopolysaccharide (LPS) by suppressing the inhibitory activity of LPS-binding protein. In J.I. 177 (7), pp. 4880–4887. DOI: 10.4049/jimmunol.177.7.4880.
[A08][A09] 2006 Westerterp, Marit; Haan, Willeke de; Berbée, Jimmy F. P.; Havekes, Louis M.; Rensen, Patrick C. N. (2006): Endogenous apoC-I increases hyperlipidemia in apoE-knockout mice by stimulating VLDL production and inhibiting LPL. In J. Lipid Res. 47 (6), pp. 1203–1211. DOI: 10.1194/jlr.M500434-JLR200.
[A08][A09] 2005 Berbée, Jimmy F. P.; van der Hoogt, Caroline C.; Sundararaman, Deepa; Havekes, Louis M.; Rensen, Patrick C. N. (2005): Severe hypertriglyceridemia in human APOC1 transgenic mice is caused by apoC-I-induced inhibition of LPL. In J. Lipid Res. 46 (2), pp. 297–306. DOI: 10.1194/jlr.M400301-JLR200.
[A08][A09] 2005 Nedelkov, Dobrin; Kiernan, Urban A.; Niederkofler, Eric E.; Tubbs, Kemmons A.; Nelson, Randall W. (2005): Investigating diversity in human plasma proteins. In Proc Natl Acad Sci USA 102 (31), pp. 10852–10857. DOI: 10.1073/pnas.0500426102.
[A08][A09] 2004 Binkley, Charles E.; Zhang, Lizhi; Greenson, Joel K.; Giordano, Thomas J.; Kuick, Rork; Misek, Dave et al. (2004): The molecular basis of pancreatic fibrosis: common stromal gene expression in chronic pancreatitis and pancreatic adenocarcinoma. In Pancreas 29 (4), pp. 254–263.


We Also Recommend