[A07] Goat Anti-Human Apolipoprotein B-100 Polyclonal Antibody, Academy Bio-medical Company, Inc.

[A07] Goat Anti-Human Apolipoprotein B-100 Polyclonal Antibody

20A-G1

Academy Bio-Medical Company, Inc.

  • $874.00


Host Species: Goat
Concentration: 1 mg/ml (OD 1.35 / 280 nm)
Antigen: Human Apolipoprotein B100
Purification: Affinity purified
Buffer: 75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2
Specificity Specifically binds to human apo B-100. Dilution for immunoblot and ELISA range: 1,000 to 80,000.
Use: The antibody can be used for detection of apo B-100 in plasma and lipoproteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.
Storage: -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.

 

*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

 

Importance

ApoB exists in human plasma in two isoforms, ApoB-48 (Chen et al., 1987) and Apo B-100 (Wei et al., 1985, Yang et al., 1986a; 1989a,b; 1990; Chen et al., 1986; Yang et al., 1990, Yang and Pownall, 1992). Apo B-100 is the major physiological ligand for the LDL receptor. Apo B-100 is a large monomeric protein, containing 4536 amino acids (m.w. 515 kDa, Yang et al., 1986b).

Apo B-100 is synthesized in the liver and is required for the assembly of VLDL. It is found in LDL and VLDL after the removal of the Apo A, E and C. Apo B-48 is present in chylomicrons and their remnants. It is essential for the intestinal absorption of dietary lipids. Apo B levels correlate with the risk of coronary disease.

The Apo B protein is directly involved in the retention of LDL with the arterial wall (Olofsson and Boren, 2012). Apo B-48 is synthesized in the small intestine. It comprises approximately half of the N-terminal region of ApoB-100 and is the result of posttranscriptional mRNA editing by a stop codon in the intestine not found in the liver.

Chen, S., G. Habib, C. Yang, Z. Gu, B. Lee, S. Weng, S. Cai, J. Deslypere, M. Rosseneu, and Al. Et. "Apolipoprotein B-48 Is the Product of a Messenger RNA with an Organ-specific In-frame Stop Codon." Science 238 (1987): 363- 66.

Olofsson, S.-O., and J. Boren. "Apolipoprotein B Secretory Regulation by Degradation." Arteriosclerosis, Thrombosis, and Vascular Biology 32 (2012): 1334-338.

Wei, C. F., S. H. Chen, C. Y. Yang, Y. L. Marcel, R. W. Milne, W. H. Li, J. T. Sparrow, A. M. Gotto, and L. Chan. "Molecular Cloning and Expression of Partial cDNAs and Deduced Amino Acid Sequence of a Carboxyl-terminal Fragment of Human Apolipoprotein B-100." Proceedings of the National Academy of Sciences 82 (1985): 7265- 269.

Yang, Chao-Yuh, and Pownall, H.J. "In Structure and Function of Plasma Apolipoproteins." Structure and Function of Apolipoproteins. Boca Raton, Fla.: CRC, 1992.

Yang, Chao-Yuh, T. W. Kim, S. A. Weng, B. R. Lee, M. L. Yang, and A. M. Gotto. "Isolation and Characterization of Sulfhydryl and Disulfide Peptides of Human Apolipoprotein B-100." Proceedings of the National Academy of Sciences 87 (1990): 5523-527.

Yang, Chao-Yuh, Z. W. Gu, S. A. Weng, T. W. Kim, S. H. Chen, H. J. Pownall, P. M. Sharp, S. W. Liu, W. H. Li, and A. M. Gotto. "Structure of Apolipoprotein B-100 of Human Low Density Lipoproteins." Arteriosclerosis, Thrombosis, and Vascular Biology 9 (1989a): 96-108.

Yang, Chao-Yuh, Zi-Wei Gu, Lawrence Chan, Henry J. Pownall, and Antonio M. Gotto. "Structure and Functional Domains of Human Apolipoprotein B-100: A Strategy to Elucidate the Structure Information of a Large Protein." Methods in Protein Sequence Analysis (1989b): 466-74.

Yang, Chao-Yuh, San-Hwan Chen, Sandra H. Gianturco, William A. Bradley, James T. Sparrow, Masako Tanimura, Wen-Hsiung Li, Doris A. Sparrow, Hans Deloof, Maryvonne Rosseneu, Fu-Shin Lee, Zi-Wei Gu, Antonio M. Gotto, and Lawrence Chan. "Sequence, Structure, Receptor-binding Domains and Internal Repeats of Human Apolipoprotein B-100." Nature 323 (1986a): 738-42.

 

Citations

[A07] 2018 Morton, Allyson M.; Koch, Manja; Mendivil, Carlos O.; Furtado, Jeremy D.; Tjønneland, Anne; Overvad, Kim et al. (2018): Apolipoproteins E and CIII interact to regulate HDL metabolism and coronary heart disease risk. In JCI insight 3 (4). DOI: 10.1172/jci.insight.98045.
[A07] 2018 Viney, Nicholas J.; Yeang, Calvin; Yang, Xiaohong; Xia, Shuting; Witztum, Joseph L.; Tsimikas, Sotirios (2018): Relationship between "LDL-C", estimated true LDL-C, apolipoprotein B-100, and PCSK9 levels following lipoprotein(a) lowering with an antisense oligonucleotide. In Journal of Clinical Lipidology 12 (3), pp. 702–710. DOI: 10.1016/j.jacl.2018.02.014.
[A07] 2017 Lin, Chi; Ryder, Lindsey; Probst, David; Caplan, Michael; Spano, Mark; LaBelle, Jeffrey (2017): Feasibility in the development of a multi-marker detection platform. In Biosensors & bioelectronics 89 (Pt 2), pp. 743–749. DOI: 10.1016/j.bios.2016.10.073.
[A07] 2017 Liu, Mingxia; Chung, Soonkyu; Shelness, Gregory S.; Parks, John S. (2017): Hepatic ABCA1 deficiency is associated with delayed apolipoprotein B secretory trafficking and augmented VLDL triglyceride secretion. In Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1862 (10 Pt A), pp. 1035–1043. DOI: 10.1016/j.bbalip.2017.07.001.
[A07] 2017 Wassef, Hanny; Bissonnette, Simon; Dufour, Robert; Davignon, Jean; Faraj, May (2017): Enrichment of Triglyceride-Rich Lipoproteins with Apolipoprotein C-I Is Positively Associated with Their Delayed Plasma Clearance Independently of Other Transferable Apolipoproteins in Postmenopausal Overweight and Obese Women. In J. Nutr. 147 (5), pp. 754–762. DOI: 10.3945/jn.116.242750.
[A07] 2016 Cheng, Dongmei; Xu, Xu; Simon, Trang; Boudyguina, Elena; Deng, Zhiyong; VerHague, Melissa et al. (2016): Very Low Density Lipoprotein Assembly Is Required for cAMP-responsive Element-binding Protein H Processing and Hepatic Apolipoprotein A-IV Expression. In J. Biol. Chem. 291 (45), pp. 23793–23803. DOI: 10.1074/jbc.M116.749283.
[A07] 2016 Lai, Yu-Sheng; Yang, Tzu-Ching; Chang, Po-Yuan; Chang, Shwu-Fen; Ho, Shu-Li; Chen, Hui-Ling; Lu, Shao-Chun (2016): Electronegative LDL is linked to high-fat, high-cholesterol diet-induced nonalcoholic steatohepatitis in hamsters. In The Journal of nutritional biochemistry 30, pp. 44–52. DOI: 10.1016/j.jnutbio.2015.11.019.
[A07] 2016 Sato, Koichi; Okajima, Fumikazu; Miyashita, Kazuya; Imamura, Shigeyuki; Kobayashi, Junji; Stanhope, Kimber L. et al. (2016): The majority of lipoprotein lipase in plasma is bound to remnant lipoproteins: A new definition of remnant lipoproteins. In Clinica chimica acta; international journal of clinical chemistry 461, pp. 114–125. DOI: 10.1016/j.cca.2016.06.020.
[A07] 2016 Weckerle, Allison; Snipes, James A.; Cheng, Dongmei; Gebre, Abraham K.; Reisz, Julie A.; Murea, Mariana et al. (2016): Characterization of circulating APOL1 protein complexes in African Americans. In J. Lipid Res. 57 (1), pp. 120–130. DOI: 10.1194/jlr.M063453.
[A07] 2014 Lee, An-Sheng; Chen, Wei-Yu; Chan, Hua-Chen; Hsu, Jing-Fang; Shen, Ming-Yi; Chang, Chia-Ming et al. (2014): Gender disparity in LDL-induced cardiovascular damage and the protective role of estrogens against electronegative LDL. In Cardiovascular diabetology 13, p. 64. DOI: 10.1186/1475-2840-13-64.
[A07] 2014 Marshall, Stephanie M.; Gromovsky, Anthony D.; Kelley, Kathryn L.; Davis, Matthew A.; Wilson, Martha D.; Lee, Richard G. et al. (2014): Acute sterol o-acyltransferase 2 (SOAT2) knockdown rapidly mobilizes hepatic cholesterol for fecal excretion. In PLoS ONE 9 (6), e98953. DOI: 10.1371/journal.pone.0098953.
[A07] 2014 Marshall, Stephanie M.; Kelley, Kathryn L.; Davis, Matthew A.; Wilson, Martha D.; McDaniel, Allison L.; Lee, Richard G. et al. (2014): Reduction of VLDL Secretion Decreases Cholesterol Excretion in Niemann-Pick C1-Like 1 Hepatic Transgenic Mice. In PLoS ONE 9 (1), e84418. DOI: 10.1371/journal.pone.0084418.
[A07] 2014 Talayero, Beatriz; Wang, Liyun; Furtado, Jeremy; Carey, Vincent J.; Bray, George A.; Sacks, Frank M. (2014): Obesity favors apolipoprotein E- and C-III-containing high density lipoprotein subfractions associated with risk of heart disease. In J. Lipid Res. 55 (10), pp. 2167–2177. DOI: 10.1194/jlr.M042333.
[A07] 2014 Yang, Tzu-Ching; Chen, Yi-Jie; Chang, Shwu-Fen; Chen, Chu-Huang; Chang, Po-Yuan; Lu, Shao-Chun (2014): Malondialdehyde mediates oxidized LDL-induced coronary toxicity through the Akt-FGF2 pathway via DNA methylation. In Journal of biomedical science 21, p. 11. DOI: 10.1186/1423-0127-21-11.
[A07] 2013 Bissonnette, Simon; Salem, Huda; Wassef, Hanny; Saint-Pierre, Nathalie; Tardif, Annie; Baass, Alexis et al. (2013): Low density lipoprotein delays clearance of triglyceride-rich lipoprotein by human subcutaneous adipose tissue. In J. Lipid Res. 54 (5), pp. 1466–1476. DOI: 10.1194/jlr.P023176.
[A07] 2013 Sumner, Anne E.; Furtado, Jeremy D.; Courville, Amber B.; Ricks, Madia; Younger-Coleman, Novie; Tulloch-Reid, Marshall K.; Sacks, Frank M. (2013): ApoC-III and visceral adipose tissue contribute to paradoxically normal triglyceride levels in insulin-resistant African-American women. In Nutrition & metabolism 10 (1), p. 73. DOI: 10.1186/1743-7075-10-73.
[A07] 2012 Avraham-Davidi, Inbal; Ely, Yona; van Pham, N.; Castranova, Daniel; Grunspan, Moshe; Malkinson, Guy et al. (2012): ApoB-containing lipoproteins regulate angiogenesis by modulating expression of VEGF receptor 1. In Nature medicine 18 (6), pp. 967–973. DOI: 10.1038/nm.2759.
[A07] 2012 Shen, Xun; Wang, Wei; Wang, Liangsu; Houde, Caroline; Wu, Weizhen; Tudor, Matt et al. (2012): Identification of genes affecting apolipoprotein B secretion following siRNA-mediated gene knockdown in primary human hepatocytes. In Atherosclerosis 222 (1), pp. 154–157. DOI: 10.1016/j.atherosclerosis.2012.02.012.
[A07] 2012 Wang, Min S.; Messersmith, Reid E.; Reed, Scott M. (2012): Membrane curvature recognition by C-reactive protein using lipoprotein mimics. In Soft matter 8 (30), pp. 7909–7918. DOI: 10.1039/C2SM25779C.
[A07] 2012 Wassef, Hanny; Salem, Huda; Bissonnette, Simon; Baass, Alexis; Dufour, Robert; Davignon, Jean; Faraj, May (2012): White Adipose Tissue Apolipoprotein C-I Secretion in Relation to Delayed Plasma Clearance of Dietary Fat in Humans. In Arterioscler Thromb Vasc Biol. 32 (11), pp. 2785–2793. DOI: 10.1161/ATVBAHA.112.300306.
[A07] 2012 Weinberg, Richard B.; Gallagher, James W.; Fabritius, Melissa A.; Shelness, Gregory S. (2012): ApoA-IV modulates the secretory trafficking of apoB and the size of triglyceride-rich lipoproteins. In J. Lipid Res. 53 (4), pp. 736–743. DOI: 10.1194/jlr.M019992.
[A07] 2011 Blade, Anna M.; Fabritius, Melissa A.; Hou, Li; Weinberg, Richard B.; Shelness, Gregory S. (2011): Biogenesis of apolipoprotein A-V and its impact on VLDL triglyceride secretion. In J. Lipid Res. 52 (2), pp. 237–244. DOI: 10.1194/jlr.M010793.
[A07] 2011 Chen, Zhu; Strack, Alison M.; Stefanni, Alice C.; Chen, Ying; Wu, Weizhen; Pan, Yi et al. (2011): Validation of Human ApoB and ApoAI Immunoturbidity Assays for Non-human Primate Dyslipidemia and Atherosclerosis Research. In J. of Cardiovasc. Trans. Res. 4 (3), pp. 373–383. DOI: 10.1007/s12265-011-9264-4.
[A07] 2010 Chung, Soonkyu; Gebre, Abraham K.; Seo, Jeongmin; Shelness, Gregory S.; Parks, John S. (2010): A novel role for ABCA1-generated large pre-beta migrating nascent HDL in the regulation of hepatic VLDL triglyceride secretion. In J. Lipid Res. 51 (4), pp. 729–742. DOI: 10.1194/jlr.M900083.
[A07] 2010 Furtado, Jeremy D.; Campos, Hannia; Sumner, Anne E.; Appel, Lawrence J.; Carey, Vincent J.; Sacks, Frank M. (2010): Dietary interventions that lower lipoproteins containing apolipoprotein C-III are more effective in whites than in blacks: results of the OmniHeart trial. In The American journal of clinical nutrition 92 (4), pp. 714–722. DOI: 10.3945/ajcn.2009.28532.
[A07] 2008 Brown, J. Mark; Bell, Thomas A.; Alger, Heather M.; Sawyer, Janet K.; Smith, Thomas L.; Kelley, Kathryn et al. (2008): Targeted Depletion of Hepatic ACAT2-driven Cholesterol Esterification Reveals a Non-biliary Route for Fecal Neutral Sterol Loss. In J. Biol. Chem. 283 (16), pp. 10522–10534. DOI: 10.1074/jbc.M707659200.
[A07] 2008 Furtado, Jeremy D.; Campos, Hannia; Appel, Lawrence J.; Miller, Edgar R.; Laranjo, Nancy; Carey, Vincent J.; Sacks, Frank M. (2008): Effect of protein, unsaturated fat, and carbohydrate intakes on plasma apolipoprotein B and VLDL and LDL containing apolipoprotein C-III: results from the OmniHeart Trial. In The American journal of clinical nutrition 87 (6), pp. 1623–1630. DOI: 10.1093/ajcn/87.6.1623.
[A07] 2008 Iqbal, Jahangir; Dai, Kezhi; Seimon, Tracie; Jungreis, Rivka; Oyadomari, Miho; Kuriakose, George et al. (2008): IRE1beta inhibits chylomicron production by selectively degrading MTP mRNA. In Cell metabolism 7 (5), pp. 445–455. DOI: 10.1016/j.cmet.2008.03.005.
[A07] 2008 Seki, Takenori; Kunichika, Tomoya; Watanabe, Kiyotaka; Orino, Koichi (2008): Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin. In Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 21 (1), pp. 61–69. DOI: 10.1007/s10534-007-9093-8.
[A07] 2007 Nagarajan, Shanmugam (2007): Anti-OxLDL IgG blocks OxLDL interaction with CD36, but promotes FcgammaR, CD32A-dependent inflammatory cell adhesion. In Immunology letters 108 (1), pp. 52–61. DOI: 10.1016/j.imlet.2006.09.008.
[A07] 2007 Temel, Ryan E.; Hou, Li; Rudel, Lawrence L.; Shelness, Gregory S. (2007): ACAT2 stimulates cholesteryl ester secretion in apoB-containing lipoproteins. In J. Lipid Res. 48 (7), pp. 1618–1627. DOI: 10.1194/jlr.M700109-JLR200.
[A07] 2007 Temel, Ryan E.; Tang, Weiqing; Ma, Yinyan; Rudel, Lawrence L.; Willingham, Mark C.; Ioannou, Yiannis A. et al. (2007): Hepatic Niemann-Pick C1-like 1 regulates biliary cholesterol concentration and is a target of ezetimibe. In The Journal of clinical investigation 117 (7), pp. 1968–1978. DOI: 10.1172/JCI30060.
[A07] 2005 Sellers, Jeremy A.; Hou, Li; Schoenberg, Daniel R.; Batistuzzo de Medeiros, Silvia R.; Wahli, Walter; Shelness, Gregory S. (2005): Microsomal triglyceride transfer protein promotes the secretion of Xenopus laevis vitellogenin A1. In J. Biol. Chem. 280 (14), pp. 13902–13905. DOI: 10.1074/jbc.M500769200.
[A07] 2004 Gallagher, James W.; Weinberg, Richard B.; Shelness, Gregory S. (2004): apoA-IV tagged with the ER retention signal KDEL perturbs the intracellular trafficking and secretion of apoB. In J. Lipid Res. 45 (10), pp. 1826–1834. DOI: 10.1194/jlr.M400188-JLR200.
[A07] 2004 Virella, Gabriel; Thorpe, Suzanne R.; Alderson, Nathan L.; Derrick, M. Brooks; Chassereau, Charlyne; Rhett, J. Matthew; Lopes-Virella, Maria F. (2004): Definition of the immunogenic forms of modified human LDL recognized by human autoantibodies and by rabbit hyperimmune antibodies. In J. Lipid Res. 45 (10), pp. 1859–1867. DOI: 10.1194/jlr.M400095-JLR200.
[A07] 2003 Shelness, Gregory S.; Hou, Li; Ledford, Aubrey S.; Parks, John S.; Weinberg, Richard B. (2003): Identification of the lipoprotein initiating domain of apolipoprotein B. In J. Biol. Chem. 278 (45), pp. 44702–44707. DOI: 10.1074/jbc.M307562200.
[A07] 2003 Yang, Chao-Yuh; Raya, Joe L.; Chen, Hsin-Hung; Chen, Chu-Huang; Abe, Yasunori; Pownall, Henry J. et al. (2003): Isolation, characterization, and functional assessment of oxidatively modified subfractions of circulating low-density lipoproteins. In Arterioscler Thromb Vasc Biol. 23 (6), pp. 1083–1090. DOI: 10.1161/01.ATV.0000071350.78872.C4.
[A07] 2001 Olin, K. L.; Potter-Perigo, S.; Barrett, P. H.; Wight, T. N.; Chait, A. (2001): Biglycan, a vascular proteoglycan, binds differently to HDL2 and HDL3: role of apoE. In Arterioscler Thromb Vasc Biol. 21 (1), pp. 129–135.

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