[A03] Goat Anti-Human Apolipoprotein AI Polyclonal Antibody, Academy Bio-medical Company, Inc.

[A03] Goat Anti-Human Apolipoprotein AI Polyclonal Antibody

11A-G2

Academy Bio-Medical Company, Inc.

  • $874.00


Host Species: Goat
Concentration: 1 mg/ml (OD 1.35 / 280 nm)
Antigen: Human Apolipoprotein AI
Purification: Affinity purified
Buffer: 75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2
Specificity Specifically binds to human apo AI. Dilution for immunoblot and ELISA range: 1,000 to 80,000.
Use: The antibody can be used for detection of apo AI in plasma and lipoproteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.
Storage: -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.

 

*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

 

Importance

Apo AI comprises approximately 70% of the protein moiety in HDL. It is a single polypeptide chain consisting of 243 amino acid residues without disulfide bound and with glutamic acid as the C-terminal residue and aspartic acid as the N-terminal residue. The molecular weight is reported to be 28 kDa (Brewer et al., 1978).

The roles of Apo AI in HDL function include reverse cholesterol transportation, lipid cholesterol binding, lecithin-cholesterol acyl transferase (LCAT) activation, and receptor binding, which is responsible for cholesterol esterification in plasma. Besides participate in cholesterol metabolism, Apo AI and HDL also suppress neutrophil activation, inhibit bacterial endotoxin, induce trypanosomal lysis, and other physiological activities. (Brouillette et al., 2001)

Apo AI levels may be inversely related to the risk of coronary disease. In previous research, Apo AI may affect diet-induced inflammation by either directly or indirectly altering lipid rafts. (Cheng et al., 2012)

Brewer, H. B., T. Fairwell, A. LaRue, R. Ronan, A. Houser, and T. J. Bronzert. “The amino acid sequence of human Apoa-I, an apolipoprotein isolated from high density lipoproteins.” Biochemical and Biophysical Research Communications 80.3 (1978): 623-30.

Brouillette, Christie G., G.m. Anantharamaiah, Jeffrey A. Engler, and David W. Borhani. "Structural Models of Human Apolipoprotein A-I: A Critical Analysis and Review." Biochimica Et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (2001): 4-46.

Cheng, Andrew M., Priya Handa, Sanshiro Tateya, Jay Schwartz, Chongren Tang, Poulami Mitra, John F. Oram, Alan Chait, and Francis Kim. "Apolipoprotein A-I Attenuates Palmitate-Mediated NF-κB Activation by Reducing Toll-Like Receptor-4 Recruitment into Lipid Rafts." PLoS ONE 7.3 (2012): e33917.

 

Citations

[A03] 2019 Sato, Megumi; Ohkawa, Ryunosuke; Low, Hann; Nishimori, Madoka; Okubo, Shigeo; Yoshimoto, Akira et al. (2019): Serum amyloid A does not affect high-density lipoprotein cholesterol measurement by a homogeneous assay. In Clinical biochemistry 63, pp. 97–101. DOI: 10.1016/j.clinbiochem.2018.10.008.
[A03] 2018 Aroner, Sarah A.; Koch, Manja; Mukamal, Kenneth J.; Furtado, Jeremy D.; Stein, James H.; Tattersall, Matthew C. et al. (2018): High‐Density Lipoprotein Subspecies Defined by Apolipoprotein C‐III and Subclinical Atherosclerosis Measures: MESA (The Multi‐Ethnic Study of Atherosclerosis). In Journal of the American Heart Association 7 (6), p. 40. DOI: 10.1161/JAHA.117.007824.
[A03] 2018 Furtado, Jeremy D.; Yamamoto, Rain; Melchior, John T.; Andraski, Allison B.; Gamez-Guerrero, Maria; Mulcahy, Patrick et al. (2018): Distinct Proteomic Signatures in 16 HDL (High-Density Lipoprotein) Subspecies. In Arterioscler Thromb Vasc Biol. 38 (12), pp. 2827–2842. DOI: 10.1161/ATVBAHA.118.311607.
[A03] 2018 Koch, Manja; DeKosky, Steven T.; Fitzpatrick, Annette L.; Furtado, Jeremy D.; Lopez, Oscar L.; Kuller, Lewis H. et al. (2018): Apolipoproteins and Alzheimer's pathophysiology. In Alzheimer's & dementia (Amsterdam, Netherlands) 10, pp. 545–553. DOI: 10.1016/j.dadm.2018.07.001.
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[A03] 2018 Satta, Nathalie; Pagano, Sabrina; Montecucco, Fabrizio; Gencer, Baris; Mach, François; Kaiser, Laurent et al. (2018): Anti-apolipoprotein A-1 autoantibodies are associated with immunodeficiency and systemic inflammation in HIV patients. In The Journal of infection 76 (2), pp. 186–195. DOI: 10.1016/j.jinf.2017.11.008.
[A03] 2017 Gillard, Baiba K.; Bassett, G. Randall; Gotto, Antonio M.; Rosales, Corina; Pownall, Henry J. (2017): Scavenger receptor B1 (SR-B1) profoundly excludes high density lipoprotein (HDL) apolipoprotein AII as it nibbles HDL-cholesteryl ester. In J. Biol. Chem. 292 (21), pp. 8864–8873. DOI: 10.1074/jbc.M117.781963.
[A03] 2017 Ikeda, Toru; Shinohata, Ryoko; Murakami, Masaaki; Hina, Kazuyoshi; Kamikawa, Shigeshi; Hirohata, Satoshi et al. (2017): A rapid and precise method for measuring plasma apoE-rich HDL using polyethylene glycol and cation-exchange chromatography: a pilot study on the clinical significance of apoE-rich HDL measurements. In Clinica chimica acta; international journal of clinical chemistry 465, pp. 112–118. DOI: 10.1016/j.cca.2016.12.016.
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[A03] 2016 Chen, Xueying; Bakillah, Ahmed; Zhou, Liye; Pan, Xiaoyue; Hoepfner, Florian; Jacob, Marrit et al. (2016): Nitrated apolipoprotein AI/apolipoprotein AI ratio is increased in diabetic patients with coronary artery disease. In Atherosclerosis 245, pp. 12–21. DOI: 10.1016/j.atherosclerosis.2015.11.021.
[A03] 2016 Ichimura, Naoya; Sato, Megumi; Yoshimoto, Akira; Yano, Kouji; Ohkawa, Ryunosuke; Kasama, Takeshi; Tozuka, Minoru (2016): High-Density Lipoprotein Binds to Mycobacterium avium and Affects the Infection of THP-1 Macrophages. In Journal of lipids 2016, p. 4353620. DOI: 10.1155/2016/4353620.
[A03] 2016 Ma, Weilie; Lin, Margarita; Ding, Hang; Lin, Guorong; Zhang, Zhizhen (2016): β-COP as a Component of Transport Vesicles for HDL Apolipoprotein-Mediated Cholesterol Exocytosis. In PloS one 11 (3), e0151767. DOI: 10.1371/journal.pone.0151767.
[A03] 2016 Negi, Priyanka; Lövgren, Janita; Malmi, Päivi; Sirkka, Nina; Metso, Jari; Huovinen, Tuomas et al. (2016): Identification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library. In Clinical biochemistry 49 (6), pp. 472–479. DOI: 10.1016/j.clinbiochem.2015.11.020.
[A03] 2016 Singh, Sasha A.; Andraski, Allison B.; Pieper, Brett; Goh, Wilson; Mendivil, Carlos O.; Sacks, Frank M.; Aikawa, Masanori (2016): Multiple apolipoprotein kinetics measured in human HDL by high-resolution/accurate mass parallel reaction monitoring. In J. Lipid Res. 57 (4), pp. 714–728. DOI: 10.1194/jlr.D061432.
[A03] 2016 Yano, Kouji; Ohkawa, Ryunosuke; Sato, Megumi; Yoshimoto, Akira; Ichimura, Naoya; Kameda, Takahiro et al. (2016): Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells. In Journal of lipids 2016, p. 9891316. DOI: 10.1155/2016/9891316.
[A03] 2016 Yassine, Hussein N.; Feng, Qingru; Chiang, Jiarong; Petrosspour, Larissa M.; Fonteh, Alfred N.; Chui, Helena C.; Harrington, Michael G. (2016): ABCA1-Mediated Cholesterol Efflux Capacity to Cerebrospinal Fluid Is Reduced in Patients With Mild Cognitive Impairment and Alzheimer's Disease. In Journal of the American Heart Association 5 (2). DOI: 10.1161/JAHA.115.002886.
[A03] 2015 Kameda, Takahiro; Ohkawa, Ryunosuke; Yano, Kouji; Usami, Yoko; Miyazaki, Akari; Matsuda, Kazuyuki et al. (2015): Effects of Myeloperoxidase-Induced Oxidation on Antiatherogenic Functions of High-Density Lipoprotein. In Journal of lipids 2015 (9), pp. 1–8. DOI: 10.1155/2015/592594.
[A03] 2015 Montecucco, Fabrizio; Braunersreuther, Vincent; Burger, Fabienne; Lenglet, Sébastien; Pelli, Graziano; Carbone, Federico et al. (2015): Anti-apoA-1 auto-antibodies increase mouse atherosclerotic plaque vulnerability, myocardial necrosis and mortality triggering TLR2 and TLR4. In Thromb Haemost 114 (08), pp. 410–422. DOI: 10.1160/TH14-12-1039.
[A03] 2015 Pagano, Sabrina; Gaertner, Hubert; Cerini, Fabrice; Mannic, Tiphaine; Satta, Nathalie; Teixeira, Priscila Camillo et al. (2015): The Human Autoantibody Response to Apolipoprotein A-I Is Focused on the C-Terminal Helix: A New Rationale for Diagnosis and Treatment of Cardiovascular Disease? In PLoS ONE 10 (7), e0132780. DOI: 10.1371/journal.pone.0132780.
[A03] 2014 Kalakech, Hussein; Hibert, Pierre; Prunier-Mirebeau, Delphine; Tamareille, Sophie; Letournel, Franck; Macchi, Laurent et al. (2014): RISK and SAFE Signaling Pathway Involvement in Apolipoprotein A-I-Induced Cardioprotection. In PLoS ONE 9 (9), e107950. DOI: 10.1371/journal.pone.0107950.
[A03] 2014 Miyazaki, Akari; Sagae, Nozomi; Usami, Yoko; Sato, Megumi; Kameda, Takahiro; Yoshimoto, Akira et al. (2014): N-homocysteinylation of apolipoprotein A-I impairs the protein’s antioxidant ability but not its cholesterol efflux capacity. In Biological Chemistry 395 (6). DOI: 10.1515/hsz-2013-0262.
[A03] 2014 Talayero, Beatriz; Wang, Liyun; Furtado, Jeremy; Carey, Vincent J.; Bray, George A.; Sacks, Frank M. (2014): Obesity favors apolipoprotein E- and C-III-containing high density lipoprotein subfractions associated with risk of heart disease. In J. Lipid Res. 55 (10), pp. 2167–2177. DOI: 10.1194/jlr.M042333.
[A03] 2014 Teixeira, Priscila Camillo; Ducret, Axel; Ferber, Philippe; Gaertner, Hubert; Hartley, Oliver; Pagano, Sabrina et al. (2014): Definition of Human Apolipoprotein A-I Epitopes Recognized by Autoantibodies Present in Patients with Cardiovascular Diseases. In J. Biol. Chem. 289 (41), pp. 28249–28259. DOI: 10.1074/jbc.M114.589002.
[A03] 2012 Chen, Zhu; Wang, Sheng-Ping; Krsmanovic, Mihajlo L.; Castro-Perez, Jose; Gagen, Karen; Mendoza, Vivienne et al. (2012): Small molecule activation of lecithin cholesterol acyltransferase modulates lipoprotein metabolism in mice and hamsters. In Metabolism 61 (4), pp. 470–481. DOI: 10.1016/j.metabol.2011.08.006.
[A03] 2012 Kameda, Takahiro; Usami, Yoko; Shimada, Saki; Haraguchi, Go; Matsuda, Kazuyuki; Sugano, Mitsutoshi et al. (2012): Determination of myeloperoxidase-induced apoAI-apoAII heterodimers in high-density lipoprotein. In Annals of clinical and laboratory science 42 (4), pp. 384–391.
[A03] 2012 Nagao, Kohjiro; Kimura, Yasuhisa; Ueda, Kazumitsu (2012): Lysine residues of ABCA1 are required for the interaction with apoA-I. In Biochimica et biophysica acta 1821 (3), pp. 530–535. DOI: 10.1016/j.bbalip.2011.06.024.
[A03] 2012 Pagano, S.; Satta, N.; Werling, D.; Offord, V.; Moerloose, P. de; Charbonney, E. et al. (2012): Anti-apolipoprotein A-1 IgG in patients with myocardial infarction promotes inflammation through TLR2/CD14 complex. In Journal of internal medicine 272 (4), pp. 344–357. DOI: 10.1111/j.1365-2796.2012.02530.x.
[A03] 2012 Shen, Xun; Wang, Wei; Wang, Liangsu; Houde, Caroline; Wu, Weizhen; Tudor, Matt et al. (2012): Identification of genes affecting apolipoprotein B secretion following siRNA-mediated gene knockdown in primary human hepatocytes. In Atherosclerosis 222 (1), pp. 154–157. DOI: 10.1016/j.atherosclerosis.2012.02.012.
[A03] 2012 Usami, Yoko; Kobayashi, Yukihiro; Kameda, Takahiro; Miyazaki, Akari; Matsuda, Kazuyuki; Sugano, Mitsutoshi et al. (2012): Identification of sites in apolipoprotein A-I susceptible to chymase and carboxypeptidase A digestion. In Bioscience reports 33 (1), pp. 49–56. DOI: 10.1042/BSR20120094.
[A03] 2011 Montecucco, Fabrizio; Vuilleumier, Nicolas; Pagano, Sabrina; Lenglet, Sébastien; Bertolotto, Maria; Braunersreuther, Vincent et al. (2011): Anti-Apolipoprotein A-1 auto-antibodies are active mediators of atherosclerotic plaque vulnerability. In European Heart Journal 32 (4), pp. 412–421. DOI: 10.1093/eurheartj/ehq521.
[A03] 2011 Rosales, Corina; Tang, Daming; Gillard, Baiba K.; Courtney, Harry S.; Pownall, Henry J. (2011): Apolipoprotein E Mediates Enhanced Plasma High-Density Lipoprotein Cholesterol Clearance by Low-Dose Streptococcal Serum Opacity Factor via Hepatic Low-Density Lipoprotein Receptors In Vivo. In Arterioscler Thromb Vasc Biol. 31 (8), pp. 1834–1841. DOI: 10.1161/ATVBAHA.111.224360.
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[A03] 2011 Vickers, Kasey C.; Palmisano, Brian T.; Shoucri, Bassem M.; Shamburek, Robert D.; Remaley, Alan T. (2011): MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. In Nat Cell Biol 13 (4), pp. 423–433. DOI: 10.1038/ncb2210.
[A03] 2011 Wang, Yanan; Berbée, Jimmy F. P.; Stroes, Erik S.; Smit, Johannes W. A.; Havekes, Louis M.; Romijn, Johannes A.; Rensen, Patrick C. N. (2011): CETP expression reverses the reconstituted HDL-induced increase in VLDL. In J. Lipid Res. 52 (8), pp. 1533–1541. DOI: 10.1194/jlr.M016659.
[A03] 2010 Carballo-Jane, Ester; Chen, Zhu; O’Neill, Edward; Wang, Jun; Burton, Charlotte; Chang, Ching H. et al. (2010): ApoA-I mimetic peptides promote pre-β HDL formation in vivo causing remodeling of HDL and triglyceride accumulation at higher dose. In Bioorganic & Medicinal Chemistry 18 (24), pp. 8669–8678. DOI: 10.1016/j.bmc.2010.09.074.
[A03] 2010 Gruaz, Lyssia; Delucinge-Vivier, Céline; Descombes, Patrick; Dayer, Jean-Michel; Burger, Danielle (2010): Blockade of T Cell Contact-Activation of Human Monocytes by High-Density Lipoproteins Reveals a New Pattern of Cytokine and Inflammatory Genes. In PLoS ONE 5 (2), e9418. DOI: 10.1371/journal.pone.0009418.
[A03] 2010 Ishimine, N.; Usami, Y.; Nogi, S.; Sumida, T.; Kurihara, Y.; Matsuda, K. et al. (2010): Identification of N-homocysteinylated apolipoprotein AI in normal human serum. In Annals of clinical biochemistry 47 (Pt 5), pp. 453–459. DOI: 10.1258/acb.2010.010035.
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[A03] 2010 Vuilleumier, Nicolas; Bas, Sylvette; Pagano, Sabrina; Montecucco, Fabrizio; Guerne, Pierre-André; Finckh, Axel et al. (2010): Anti-apolipoprotein A-1 IgG predicts major cardiovascular events in patients with rheumatoid arthritis. In Arthritis and rheumatism 62 (9), pp. 2640–2650. DOI: 10.1002/art.27546.
[A03] 2009 Kim, Soo In; Shin, Duckhyang; Lee, Hyeon; Ahn, Byung-Yoon; Yoon, Yeup; Kim, Meehyein (2009): Targeted delivery of siRNA against hepatitis C virus by apolipoprotein A-I-bound cationic liposomes. In Journal of hepatology 50 (3), pp. 479–488. DOI: 10.1016/j.jhep.2008.10.029.
[A03] 2009 Vries-van der Weij, Jitske de; Haan, Willeke de; Hu, Lihui; Kuif, Maarten; Oei, H. Ling D. W.; van der Hoorn, José W. A. et al. (2009): Bexarotene Induces Dyslipidemia by Increased Very Low-Density Lipoprotein Production and Cholesteryl Ester Transfer Protein-Mediated Reduction of High-Density Lipoprotein. In Endocrinology 150 (5), pp. 2368–2375. DOI: 10.1210/en.2008-1540.
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[A03] 2008 Deng, Ting; Ji, Wei; Lian, Ji-Hong; Guo, Lei; Hu, Wei-Rong; Qian, Ming; Gong, Bang-qiang (2008): Identifying Natural Derived Upregulators of Human ApoA-I Expression via a Cell-Based Drug Screening System. In Pharmaceutical Biology 46 (9), pp. 610–615. DOI: 10.1080/13880200802179584.
[A03] 2008 He, Yubin; Greene, Diane J.; Kinter, Michael; Morton, Richard E. (2008): Control of cholesteryl ester transfer protein activity by sequestration of lipid transfer inhibitor protein in an inactive complex. In J. Lipid Res. 49 (7), pp. 1529–1537. DOI: 10.1194/jlr.M800087-JLR200.
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[A03] 2007 Kim, Soo In; Shin, Duckhyang; Choi, Tae Hyun; Lee, Jong Chan; Cheon, Gi-Jeong; Kim, Ki-Yong et al. (2007): Systemic and specific delivery of small interfering RNAs to the liver mediated by apolipoprotein A-I. In Molecular therapy : the journal of the American Society of Gene Therapy 15 (6), pp. 1145–1152. DOI: 10.1038/sj.mt.6300168.
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[A03] 2006 Thompson, Patricia A.; Kitchens, Richard L. (2006): Native high-density lipoprotein augments monocyte responses to lipopolysaccharide (LPS) by suppressing the inhibitory activity of LPS-binding protein. In J.I. 177 (7), pp. 4880–4887. DOI: 10.4049/jimmunol.177.7.4880.
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[A03] 2004 Gallagher, James W.; Weinberg, Richard B.; Shelness, Gregory S. (2004): apoA-IV tagged with the ER retention signal KDEL perturbs the intracellular trafficking and secretion of apoB. In J. Lipid Res. 45 (10), pp. 1826–1834. DOI: 10.1194/jlr.M400188-JLR200.
[A03] 2004 Pastore, Lucio; Belalcazar, L. Maria; Oka, Kazuhiro; Cela, Racel; Lee, Brendan; Chan, Lawrence; Beaudet, Arthur L. (2004): Helper-dependent adenoviral vector-mediated long-term expression of human apolipoprotein A-I reduces atherosclerosis in apo E-deficient mice. In Gene 327 (2), pp. 153–160. DOI: 10.1016/j.gene.2003.11.024.
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[A03] 2001 Yuhanna, I. S.; Zhu, Y.; Cox, B. E.; Hahner, L. D.; Osborne-Lawrence, S.; Lu, P. et al. (2001): High-density lipoprotein binding to scavenger receptor-BI activates endothelial nitric oxide synthase. In Nature medicine 7 (7), pp. 853–857. DOI: 10.1038/89986.

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