[P19] Malondialdehyde- modified Human Albumin (MDA - Albumin)10P-MD-AL102
|Concentration:||1 mg/ml (OD 1.35 / 280 nm)|
|Source:||Malondialdehyde modification from Human Albumin.|
|Purity:||??? 98% by SDS-PAGE|
|Buffer:||In 75 mM Sodium Phosphate, 75 m M NaCl, 0.02 % NaN3, 1 mM EDTA, p H 7.4.|
|Storage:||-20??C for long-term and short-term storage. Aliquot to avoid repeated freezing and thawing.|
*The products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.
Oxidative damage includes oxidative modification of cellular macromolecules, induction of cell death by apoptosis or necrosis, as well as structural tissue damage. Of the many biological targets of oxidative stress, lipids are the most involved class of biomolecules. Lipid oxidation gives rise to a number of secondary products of polyunsaturated fatty acid peroxidation.
Malondialdehyde (MDA) is the principal and most studied product. Consistent evidence reveals the reaction between MDA and cellular macromolecules such as proteins, RNA and DNA (Valenzuela, 1991). Numerous experiments have shown that MDA readily modifies proteins (Nair, 1986). MDA reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine which may be mutagenic and these can be quantified in several human tissues (Marnett, 1999).This aldehyde is a highly toxic molecule and should be considered as a marker of lipid peroxidation. The interaction with DNA and proteins has often been referred to as potentially mutagenic and atherogenic (Rio et al., 2005).
L.J. Marnett, Lipid peroxidation?€?DNA damage by malondialdehyde, Mutat Res, 424 (1999), pp. 83?€?95
V. Nair, C.S. Cooper, D.E. Vietti, G.A. Turner, The chemistry of lipid peroxidation metabolites: crosslinking reactions of malondialdehyde, Lipids, 21 (1986), pp. 6?€?10
Rio, Daniele Del, Amanda J. Stewart, and Nicoletta Pellegrini. "A Review of Recent Studies on Malondialdehyde as Toxic Molecule and Biological Marker of Oxidative Stress."??Nutrition, Metabolism and Cardiovascular Diseases??15.4 (2005): 316-28.??
A. Valenzuela, The biological significance of malondialdehyde determination in the assessment of tissue oxidative stress, Life Sci, 48 (1991), pp. 301?€?309
|[P19]||2007||Odhiambo, Adam; Perlman, David H.; Huang, Hua; Costello, Catherine E.; Farber, Harrison W.; Steinberg, Martin H. et al. (2007): Identification of oxidative post-translational modification of serum albumin in patients with idiopathic pulmonary arterial hypertension and pulmonary hypertension of sickle cell anemia. In Rapid communications in mass spectrometry : RCM 21 (14), pp.??2195?€?2203. DOI: 10.1002/rcm.3074.|