[H02] HRP-conjugated Sheep Anti-Human Apolipoprotein (a)10H-S1a
|Concentration:||0.8 mg/ml (OD 1.35 / 280 nm)|
|Antigen:||Human Apolipoprotein (a)|
|Buffer:||50 mM PBS, 0.1 M NaCl, and 0.01% Thiomersal, pH 7.4.|
|Specificity||Specifically binds to human Lp (a) and apo (a). No cross- reaction to Apo B-100/48; minor to plasminogen. Dilution for immunoblot and ELISA range: 1,000 to 8,000.|
|Use:||The antibody can be used for detection of human albumin in plasma and lipoproteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.|
|Storage:||-20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.|
|Form:||Freeze dried powder|
|Stabilizer:||10 mg / ml Bovine Serum Albumin.|
Freeze-dried product should be stored refrigerated until opened. After opening, restore to suggested ml volume with distilled water. If it is not completely clear after standing for 1-2 hours at room temperature, centrifuge the product. It is stable for several weeks at 4°C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20°C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.
*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.
The Lipoprotein (a) [Lp(a)] structure is similar to that of low density lipoprotein (LDL), regarding size and lipid composition of the particles and the presence of apolipoprotein B-100 (apo B-100). In addition to apo B-100, Lp(a) has a second protein, apolipoprotein (a) [apo (a)], which the molecular weight of that glycoprotein varies widely from 400 to 700 kDa. (Maranhão et al., 2013)
Previous research has shown that apo (a) has a similar structure with plasminogen and contains a serine protease domain and two types of plasminogen-like kringle domains. (McLean et al., 1987) The plasma concentration of human lipoprotein[a], Lp[a], is highly correlated with coronary artery disease. (Gaubatz et al., 1990)
Gaubatz, J.W., K. I. Ghanem, J. Jr. Guevara, M. L. Nava, W. Patsch, and J. D. Morrisett. “Polymorphic forms of human apolipoprotein[a]: inheritance and relationship of their molecular weights to plasma levels of lipoprotein[a].” J. Lipid Res. 31.4 (1990):603-13.
Mclean, John W., James E. Tomlinson, Wun-Jing Kuang, Dan L. Eaton, Ellson Y. Chen, Gunther M. Fless, Angelo M. Scanu, and Richard M. Lawn. "CDNA Sequence of Human Apolipoprotein(a) Is Homologous to Plasminogen." Nature 330.6144 (1987): 132-37.
Maranhão, Raul Cavalcante, Priscila Oliveira Carvalho, Celia Cassaro Strunz, and Fulvio Pileggi. "Lipoprotein (a): Structure, Pathophysiology and Clinical Implications." Arquivos Brasileiros De Cardiologia 103.1 (2014): 76-84.