[B01] Biotinylated Goat Anti-Human Albumin, Academy Bio-medical Company, Inc.

[B01] Biotinylated Goat Anti-Human Albumin

AL10B-G1a

Academy Bio-Medical Company, Inc.

  • $212.00


Host Species: Goat
Concentration: 1 mg/ml (OD 1.35 / 280 nm)
Antigen: Human albumin
Purification: Affinity purified
Buffer: 75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2
Specificity Specifically binds to human albumin. Dilution for immunoblot & ELISA range: 1,000 to 80,000. Centrifuge before use.
Use: The antibody can be used for detection of human albumin in plasma and lipoproteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.
Storage: -20??C for long-term storage, 4??C for short- term storage. Aliquot to avoid repeated freezing and thawing.
Form: Freeze dried powder
Stabilizer: 10 mg / ml Bovine Serum Albumin.

Reconstitution

and Storage:

Freeze-dried product should be stored refrigerated until opened. After opening, restore to suggested ml volume with distilled water. If it is not completely clear after standing for 1-2 hours at room temperature, centrifuge the product. It is stable for several weeks at 4??C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20??C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.

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*These products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

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Importance

Human albumin constitutes around 55% of the protein present in the plasma of normal healthy individuals. It is a single polypeptide chain of 585 amino acids with a molecular weight of 66 kDa.

The functions of human serum albumin include maintaining oncotic pressure; binding substances or drugs; regulating endogenous substances, metabolism; plasma buffer; antioxidant function; maintaining microvascular integrity; anticoagulant effects; inflammatory/anti-inflammatory effects; cell-signaling process. (Nicholson et al., 2000; Evans, 2002) Albumin also has functions that may play a critical role and have a great impact on different types of diseases, including hypo-albumin aemia and peripheral oedema. (Caironi et al. 2009)

Caironi, Pietro, and Luciano Gattinoni. ?€?The clinical use of albumin: the point of view of a specialist in intensive care.?€? Blood Tranfusion 7.4 (2009): 259-67.

Evans, T. W. "Review Article: Albumin as a Drug-biological Effects of Albumin Unrelated to Oncotic Pressure."??Alimentary Pharmacology and Therapeutics??16 (2002): 6-11.

Nicholson, J. P., M. R. Wolmarans, and G. R. Park. "The Role of Albumin in Critical Illness."??British Journal of Anaesthesia??85.4 (2000): 599-610.??

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